{"created":"2023-05-15T09:36:25.424765+00:00","id":839,"links":{},"metadata":{"_buckets":{"deposit":"72ebd193-3ab3-40c5-8368-56af044d109f"},"_deposit":{"created_by":13,"id":"839","owners":[13],"pid":{"revision_id":0,"type":"depid","value":"839"},"status":"published"},"_oai":{"id":"oai:seitoku.repo.nii.ac.jp:00000839","sets":["59:57:147"]},"author_link":["1948","1191","1949","1947","1946"],"control_number":"839","item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"83","bibliographicPageStart":"77","bibliographicVolumeNumber":"30","bibliographic_titles":[{"bibliographic_title":"研究紀要"},{"bibliographic_title":"Bulletin of Seitoku University, Bulletin of Seitoku University Junior College","bibliographic_titleLang":"en"}]}]},"item_10002_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"PDF","subitem_description_type":"Other"}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"要旨\n　【目的】本研究では牛乳清中のLPS/TLR ４シグナル抑制分子の検索を目的とした。【方法】TLR ４/CD14/MD2-HEK293 細胞及びRAW264.7 細胞を用い、LPS/TLR ４シグナルに対する乳清の阻害効果を調べた。【結果】乳清溶液では、LPS/TLR ４シグナルを介した、TLR ４/CD14/MD2-HEK293 細胞でのSEAP 発現、及びRAW 264.7 細胞接着の阻害が確認された。さらに、Superdex 75 10/300 GL クロマトグラフィーにより、LPS 刺激で誘導されるTLR ４/CD14/MD2-HEK293 細胞のSEAP 発現、及びRAW264.7 細胞からのTRAP 陽性多核巨細胞の形成を抑制する、proteinase K 感受性の分子量約7,000 の画分が得られた。【結論】乳清中に、従来報告のない分子量約7,000のLPS/TLR ４シグナル抑制分子の存在を見出した。","subitem_description_type":"Abstract"},{"subitem_description":"Abstract\n　【Objective】The purpose of this study was to research LPS/TLR４ signal suppressor molecules in cow whey.　【Procedure】TLR４/CD14/MD2-HEK293 and RAW264.7 cells were used to examine the inhibitory effect of whey on LPS/TLR４ signal transduction.【Results】In whey lysates, inhibition of SEAP expression in TLR４/ CD14/MD2-HEK293 cells via LPS /TLR４ signals, as well as inhibition of RAW264.7 cell adhesion via LPS/TLR４ signals was confirmed.　Furthermore, Superdex 75 10/300 GL chromatography yields a proteinase K sensitive fraction with a molecular weight of approximately 7,000 that suppresses LPS stimulated expression of SEAP in TLR４/CD14/MD2-HEK293 cells and also suppresses LPS stimulated formation of TRAP-positive multinuclear giant cells from RAW264.7 cells.【Conclusion】In this study, we discovered the existence of LPS/TLR４ signal suppressor molecule of about 7,000 in cow whey.","subitem_description_type":"Abstract"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"聖徳大学"}]},"item_10002_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA12621204","subitem_source_identifier_type":"NCID"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"横山, 嘉子","creatorNameLang":"ja"},{"creatorName":"ヨコヤマ, ヨシコ","creatorNameLang":"ja-Kana"},{"creatorName":"YOKOYAMA, Yoshiko","creatorNameLang":"en"}],"familyNames":[{"familyName":"横山","familyNameLang":"ja"},{"familyName":"ヨコヤマ","familyNameLang":"ja-Kana"},{"familyName":"YOKOYAMA","familyNameLang":"en"}],"givenNames":[{"givenName":"嘉子","givenNameLang":"ja"},{"givenName":"ヨシコ","givenNameLang":"ja-Kana"},{"givenName":"Yoshiko","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1946","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"藤原, しのぶ","creatorNameLang":"ja"},{"creatorName":"フジハラ, シノブ","creatorNameLang":"ja-Kana"},{"creatorName":"FUJIHARA, Shinobu","creatorNameLang":"en"}],"familyNames":[{"familyName":"藤原","familyNameLang":"ja"},{"familyName":"フジハラ","familyNameLang":"ja-Kana"},{"familyName":"FUJIHARA","familyNameLang":"en"}],"givenNames":[{"givenName":"しのぶ","givenNameLang":"ja"},{"givenName":"シノブ","givenNameLang":"ja-Kana"},{"givenName":"Shinobu","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1191","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"岩崎, 有希","creatorNameLang":"ja"},{"creatorName":"イワサキ, ユキ","creatorNameLang":"ja-Kana"},{"creatorName":"IWASAKI, Yuki","creatorNameLang":"en"}],"familyNames":[{"familyName":"岩崎","familyNameLang":"ja"},{"familyName":"イワサキ","familyNameLang":"ja-Kana"},{"familyName":"IWASAKI","familyNameLang":"en"}],"givenNames":[{"givenName":"有希","givenNameLang":"ja"},{"givenName":"ユキ","givenNameLang":"ja-Kana"},{"givenName":"Yuki","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1947","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"白石, 弘美","creatorNameLang":"ja"},{"creatorName":"シライシ, ヒロミ","creatorNameLang":"ja-Kana"},{"creatorName":"SHIRAISHI, Hiromi","creatorNameLang":"en"}],"familyNames":[{"familyName":"白石","familyNameLang":"ja"},{"familyName":"シライシ","familyNameLang":"ja-Kana"},{"familyName":"SHIRAISHI","familyNameLang":"en"}],"givenNames":[{"givenName":"弘美","givenNameLang":"ja"},{"givenName":"ヒロミ","givenNameLang":"ja-Kana"},{"givenName":"Hiromi","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1948","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"加納, 和孝","creatorNameLang":"ja"},{"creatorName":"カノウ, カズタカ","creatorNameLang":"ja-Kana"},{"creatorName":"KANO, Kazutaka","creatorNameLang":"en"}],"familyNames":[{"familyName":"加納","familyNameLang":"ja"},{"familyName":"カノウ","familyNameLang":"ja-Kana"},{"familyName":"KANO","familyNameLang":"en"}],"givenNames":[{"givenName":"和孝","givenNameLang":"ja"},{"givenName":"カズタカ","givenNameLang":"ja-Kana"},{"givenName":"Kazutaka","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1949","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-05-18"}],"displaytype":"detail","filename":"201930_077-083.pdf","filesize":[{"value":"529.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"201930_077-083","url":"https://seitoku.repo.nii.ac.jp/record/839/files/201930_077-083.pdf"},"version_id":"f82a3c0e-edec-451d-8c6d-46578c308e98"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"乳清","subitem_subject_scheme":"Other"},{"subitem_subject":"Toll 様受容体４（TLR ４）","subitem_subject_scheme":"Other"},{"subitem_subject":"抗炎症性","subitem_subject_scheme":"Other"},{"subitem_subject":"破骨細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"whey","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Toll-like receptor ４（TLR ４）","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"anti-inflammatory","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"osteoclasts","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"乳清中のLPS/TLR ４シグナル抑制分子の検索","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"乳清中のLPS/TLR ４シグナル抑制分子の検索","subitem_title_language":"ja"},{"subitem_title":"Research of LPS/TLR4 Signal Transduction Suppressor Molecules in Cow Whey","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"13","path":["147"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-05-18"},"publish_date":"2020-05-18","publish_status":"0","recid":"839","relation_version_is_last":true,"title":["乳清中のLPS/TLR ４シグナル抑制分子の検索"],"weko_creator_id":"13","weko_shared_id":-1},"updated":"2024-06-03T01:46:45.357906+00:00"}